https://nova.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:50840 Wed 28 Feb 2024 16:33:00 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:44648 2NPs) is on the increase, and so the number of studies dedicated to describing this material's biological effects. Previous studies have presented results indicating the controversial impact of TiO₂NPs on cell fate regarding death and survival. We speculate that this may be due to focusing on each of the subject cells as an isolated individual. In this study, we made a difference by looking at the subject cells as an interrelated population. Specifically, we exposed mesenchymal stem cells (MSCs) to TiO₂NPs and observed cell death and stimulation of proliferation among the cell population. Our data shows that the exposure to TiO₂NPs initiated autophagy, which led to an increase in extracellular Wnt protein levels and increased Wnt/GSK3β/β-catenin/cyclin D1 signalling in the cell population. Autophagy inhibitor repressed the effects of TiO₂NPs, which indicates that ß-catenin regulation was dependent on TiO₂NPs-induced autophagy. The inhibition of β-catenin resulted in dysregulation of cyclin D1 protein expression level. In conclusion, following exposure to TiO₂NPs, MSCs undergo autophagy, which induces cell proliferation among the cell population by upregulation of cyclin D1 through the Wnt/GSK3β/β-catenin pathway.]]> Wed 19 Oct 2022 09:00:38 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:26646 Wed 11 Apr 2018 17:03:53 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20455 Wed 11 Apr 2018 16:49:10 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:45559 n = 27) was characterised by immunohistochemistry and by mRNA analyses. Stroma was identified by immunostaining of stromal matrix collagen type I and proliferating cells were identified by Ki67 expression. The cortical and medullar volume expanded across gestation, with the rate of cortical expansion slowing over time. During gestation, the proportion of stroma in the cortex and total volume in the cortex significantly increased (P < 0.05). The proliferation index and numerical density of proliferating cells in the stroma significantly decreased (P < 0.05), whereas the numerical density of cells in the stroma did not change (P > 0.05). The expression levels of 12 genes out of 18 examined, including osteoglycin (OGN) and lumican (LUM), were significantly increased later in development (P < 0.05) and the expression of many genes was positively correlated with other genes and with gestational age. Thus, the rate of cortical stromal expansion peaked in early gestation due to cell proliferation, whilst late in development expression of extracellular matrix genes increased.]]> Tue 01 Nov 2022 13:09:00 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:23968 Thu 17 Mar 2022 14:35:49 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:9702 Sat 24 Mar 2018 08:34:38 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:49577 Mon 22 May 2023 10:51:01 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:42305 n = 9/group). Statistically different levels of expression were determined (P < 0.05). All nine miRNA mimics significantly affected the proliferation rates of HTR-8/SVneo cells. Five of the miRNA mimics (miR-181a-5p (predicted to target: renin (REN), angiotensin converting enzyme (ACE)), miR-378 (REN, ACE), miR-663 (REN), miR-483-3p (ACE, ACE2, angiotensinogen (AGT), angiotensin II type 1 receptor (AGTR1) and miR-514 (AGT)) were associated with a dose-dependent reduction in cell proliferation. Seven of the mimics significantly decreased expression of at least one of their predicted target RAS mRNAs. Our study shows that miRNAs targeting placental RAS mRNAs play a role in controlling trophoblast proliferation. As placentation is largely a process of proliferation, changes in expression of these miRNAs may be partly responsible for the expression of the placental RAS, proliferation and placentation.]]> Mon 22 Aug 2022 09:00:28 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:41224 Fri 29 Jul 2022 10:25:19 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:52684 Fri 20 Oct 2023 09:58:07 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:35545 Fri 03 Dec 2021 10:33:29 AEDT ]]>